Basic Info.
Product Description
Product Description
Product name: Monkeypox Virus (MPV) DNA Detection Kit
*Monkeypox is a viral zoonotic disease caused by monkeypox virus. It occurs primarily in tropical rainforest areas
of Central and West Africa and is occasionally exported to other regions.
Usage Steps
1. Reagent Preparation(performed at "reagent preparation region")
1.1 Take out each componentsfrom the diagnostic kitand placethemat room temperature. Allow thereagents to equilibrateatroom temperature, then vortex each of themrespectively forlater use.
1.2 According to thequantityof test specimens, MPV Positive Control, MPVNegative Control,and Internal control (IC), pipetteappropriate quantity of MPVPCR Mix andMPVEnzymeMix ( MPV PCR Mix 15 μl/test +MPV EnzymeMix 4 μl/test +Internal control (IC) 1 μl/test), mix them thoroughlytomake a PCR-Mastermix, centrifuge itinstantaneously forlater use.
1.3 Transfer the above-prepared reagents to the"specimen processing region" for later use.
2. Processing and loading ofspecimens (performed at "specimenprocessing region")
2.1 Thisdiagnostic kit does not include Viral RNA&DNA Extraction Kit. Itisrecommended to use Viral RNA&DNA ExtractionKit produced by Changsha Renji Medical Equipment Co., Ltd. to extract viral DNA. The specific operation is in accordancewith its instructions.
2.2 Add 20μl PCR-Mastermix into PCR reaction tube with 5μl above processed sample, MPV Positive Control and MPV NegativeControl, and cap the tube. Carry outfluorescence quantitativePCR detection on fluorescencePCR instrument.
3.PCRAmplification (performed at "nucleic acidamplificationarea")
3.1 Place PCRreaction tubes into thespecimenwells of the amplification equipment. SetupMPVPositive Control, MPV NegativeControl and specimens to be tested in the corresponding sequence and input specimen name.
3.2 Set cycle parameters according to the following table for PCR amplification.
Other Detection Kit
Company Profile
Changsha Renji Medical Equipment Co., Ltd. is a medical consumable products industrial chain company with developing and manufacturing a complete product line for molecular laboratories, ranging from upstream consumable medical products, nucleic acid extraction kits and full automatic instruments to downstream real time PCR diagnostic & research kits and fluorescence systems.
Based on innovative R&D center in Changsha, China, Renji understands its capability and social responsibility to offer molecular diagnostic products consistent in quality, accurate and reliable in test results, easy to use and accessible to laboratories of all levels despite their variety in lab environment. Renji is gradually winning its reputation all over the world through growing influence on popularizing RT-PCR technologies towards they are supposed to be as affordable and accessible human health care means.
In the future ,"RENJI" will be committed to integrating the development of enterprise into the national health industry development strategy, building a new pattern of innovation and development and building a new high ground of modern medical plastics industry chain. "RENJI" will bear in mind the original intention that quality is the survival foundation of enterprise and product is equal to people' s charter to build "RENJI" brand and make progress hands in hands with counterparts at home and abroad for contribution to the human health.
Certifications
Packing & Shipping
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