Basic Info.
Origin
China
HS Code
300590900
Production Capacity
500000
Product Description
Product Description
Monkeypox Virus Real Time PCR Kit
Monkey pox is a zoonotic virus, causing symptoms similar to small pox, though less severe clinically. It belongs to orthopoxvirus genus of Poxviridae family. Two clades of Monkeypox are known: the West African and the Congo Basin (Central African). It can be transmitted from one person to other through close contact with lesions, body fluids, droplets and contaminated material such as linen. The incubation period for infection is usually 6-13 days, however it can vary from 5 to 21 days. As per WHO, possible symptoms may include headache, fever, swollen lymph nodes, weakness, muscle and body ache. The Monkeypox virus can be detected by real time polymerase chain reaction, transmission electron microscopy and immunohistochemistry.
Intended Use
We are specialized in the medical equipment industry.The Monkeypox Virus Real Time PCR kit is used for the detection of Monkeypox virus in serum or lesion exudate samples by using real time PCR systems.
Product Performance Index
1. LOD: The limit of detection is 200 copies/ml.
2. Precision: Coefficient of variation (CV%) of precision Ct value within batch ≤3%.
3. Specificity: There is no cross reaction between the kit and positive samples, such as Smallpox Virus, Cowpox Virus,
Vaccinia Virus etc.
Usage Steps
1. Reagent Preparation(performed at "reagent preparation region")
1.1 Take out each componentsfrom the diagnostic kitand placethemat room temperature. Allow thereagents to equilibrateatroom temperature, then vortex each of themrespectively forlater use.
1.2 According to thequantityof test specimens, MPV Positive Control, MPVNegative Control,and Internal control (IC), pipetteappropriate quantity of MPVPCR Mix andMPVEnzymeMix ( MPV PCR Mix 15 μl/test +MPV EnzymeMix 4 μl/test +Internal control (IC) 1 μl/test), mix them thoroughlytomake a PCR-Mastermix, centrifuge itinstantaneously forlater use.
1.3 Transfer the above-prepared reagents to the"specimen processing region" for later use. 2. Processing and loading ofspecimens (performed at "specimenprocessing region")
2.1 Thisdiagnostic kit does not include Viral RNA&DNA Extraction Kit. Itisrecommended to use Viral RNA&DNA ExtractionKit produced by Changsha Renji Medical Equipment Co., Ltd. to extract viral DNA. The specific operation is in accordancewith its instructions.
2.2 Add 20μl PCR-Mastermix into PCR reaction tube with 5μl above processed sample, MPV Positive Control and MPV NegativeControl, and cap the tube. Carry outfluorescence quantitativePCR detection on fluorescencePCR instrument.
3.PCRAmplification (performed at "nucleic acidamplificationarea")
3.1 Place PCRreaction tubes into thespecimenwells of the amplification equipment. SetupMPVPositive Control, MPV NegativeControl and specimens to be tested in the corresponding sequence and input specimen name.
3.2 Set cycle parameters according to the following table for PCR amplification.
Company Profile
Changsha Renji Medical Equipment Co.,Ltd is a medical supply company specilizing in products series, such as disposable virus specimen collection sampler and Saliva collection kits for PCR test. Vacuum blood collection tubes and other medical consumables. We are direct manufacturer based on Changsha City Hunan Province. And many of our staffs are gene experts and professional doctors who have plenty of experience in this industry. Our products have been approved by many certificates. Such as CE, SGS,ISO and so on.
Production Process
Certificate
