*Monkeypox is a viral zoonotic disease caused by monkeypox virus. It occurs primarily in tropical       rainforest areas of Central and West Africa and is occasionally exported to other regions.

1. Reagent Preparation(performed at "reagent preparation region")
1.1 Take out each componentsfrom the diagnostic kitand placethemat room temperature. Allow thereagents to equilibrateatroom temperature, then vortex each of themrespectively forlater use.
1.2 According to thequantityof test specimens, MPV Positive Control, MPVNegative Control,and Internal control (IC), pipetteappropriate quantity of MPVPCR Mix andMPVEnzymeMix ( MPV PCR Mix 15 μl/test +MPV EnzymeMix 4 μl/test +Internal control (IC) 1 μl/test), mix them thoroughlytomake a PCR-Mastermix, centrifuge itinstantaneously forlater use.

1.3 Transfer the above-prepared reagents to the"specimen processing region" for later use.

2. Processing and loading ofspecimens (performed at "specimenprocessing region")
2.1 Thisdiagnostic kit does not include Viral RNA&DNA Extraction Kit. Itisrecommended to use Viral RNA&DNA ExtractionKit produced by Changsha Renji Medical Equipment Co., Ltd. to extract viral DNA. The specific operation is in accordancewith its instructions.
2.2 Add 20μl PCR-Mastermix into PCR reaction tube with 5μl above processed sample, MPV Positive Control and MPV NegativeControl, and cap the tube. Carry outfluorescence quantitativePCR detection on fluorescencePCR instrument.

3.PCRAmplification (performed at "nucleic acidamplificationarea")
3.1 Place PCRreaction tubes into thespecimenwells of the amplification equipment. SetupMPVPositive Control, MPV NegativeControl and specimens to be tested in the corresponding sequence and input specimen name.
3.2 Set cycle parameters according to the following table for PCR amplification.

Changsha Renji Medical Equipment Co.,Ltd is a medical supply company specilizing in  products series, such as disposable virus specimen collection sampler and  Saliva collection kits for PCR test. Vacuum blood collection tubes and other medical consumables. We are direct manufacturer based on Changsha City Hunan Province. And many of our staffs are gene experts and professional doctors who have plenty of experience in this industry. Our products have been approved by many certificates. Such as CE, SGS,ISO and so on.