--Prediction indicators for hand-foot-mouth disease (HFMD)

[Background]

Enteroviruses that cause HFMD include more than 30 kinds of viruses, such as poliovirus, coxsackie virus, ECHO virus and New enterovirus. Among which EV71 and CA16 enteroviruses are the most common pathogens, and EV71 infection is the main cause of severe cases and deaths.

[Clinical significance]

Carry out enterovirus nucleic acid test in the infant eruption period, and even before the eruption showing continuous fever, poor mental health, vomiting and other symptoms to clarify whether the patient has enterovirus infection to, so as to early prevention, early treatment
For severe cases with atypical rash, clinical diagnosis is difficult, and it is more necessary to carry out etiological diagnosis to deter- mine whether there is enterovirus infection
HFMD has a fast onset rate, and the detection of enterovirus nucleic acid can achieve early intervention and early blocking.

[Testing Principle] 
Multiple fluorescent PCR was used to simultaneously detect EV71, CA16 and other enteroviruses with different fluorescent probes in a single tube one time.

[Advantage]

• Multi-channel detection technology: Confirmation and typing were completed simultaneously by labeling three fluorescent probes;
• Simple and Convenient: Complete Sample processing within half an hour. One-step reverse transcriptase amplification method is easy to operate and avoid cross contamination;
• Original MS2 internal standard: Monitor the whole process of nucleic acid extraction and detection, which is beneficial to eliminate the false negative in the reaction, and make the result more reliable;
• High Specificity: No cross-reaction happened with other viruses with the same infection site or similar infection symptoms, and the specificity was more than 95%.
• Fast and efficient: Multiple enteroviruses were detected in a single PCR reaction time.